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Objective To assess the clinical application value of color Doppler ultrasound guided puncture in peripherally inserted central catheter (PICC). Methods Thirty-two patients needed long-term intravenous infusion underwent PICC. Color Doppler ultrasound was used to select the puncture vascular,the best puncture point and angle, and the entire process was monitored and guided dynamically, and the initial position of the catheter tip was located. Results Color Doppler ultrasound-guided puncture was successful in all 32 patients, and the successful rate was 100%. The guided puncture time was 22 s to 19 min, and the first puncture succeeded in 30 patients (93.75%). Conclusion Color Doppler ultrasound-guided puncture in PICC can obviously raise the success rate of puncture, shorten puncture time and reduce the complications. It is an easy, safe and certain method.
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Objective To investigate the protective effect of transfecting bone morphogenetic protein-7 (BMP-7) on cultured neonatal rat cardiomyocytes against hypoxia-reoxygenation injury. Methods Neonatal rat cardiomyocytes were cultured. The pcDNA-rrBMP7 was introduced into cardiomyocytes by Fugene 6.0 transfection method. The cardiomyocytes were divided into three groups: control group (group C), hypoxia-reoxygenation group (group HR) and gene transfecting group (group BT). Trypan blue exclusion test was performed to detect cell viability. The activity of lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) was assayed to evaluate cell injury. For evaluating the cell antioxidant ability, the activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) were determined by colorimetric assay. Fluo-3 labeling method and confocal laser scanning microscopy were used to observe the change in intracellular calcium. Results The results showed that after 120 min of simulated ischemia followed by 240 min of reperfusion, cell pulsation rate was decreased, the activity of LDH, CPK and the trypan blue uptake rate were increased. As compared with the group C, SOD activity decreased and the content of MDA increased in Group HR. Compared with Group HR, the SOD activity increased and the content of MDA decreased in group BT. Treatment with BMP-7 gene transfecting led to a decrease of i content in cardiomyocytes, showing that overloading of i induced by hypoxia-reoxygenation was prevented (P
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Objective To determine the effects of propofol on c fos gene expression in the different brain regions following stress in rats Methods Twenty one male Wistar rats (12 18 weeks) weighing 260 300g were randomly divided into three groups of seven animals each: control group(C); electrical stimulation group(S) and propofol group(P) The animals were anesthetized with pentobarbital sodium 40mg?kg -1 Normal saline 2ml (group C and S) or propofol 10mg?kg -1 (group P) was injected intraperitoneally (ip) 5 min after ip injection hindpaw of the animals in group S and P was electrically stimulated with 2 mA direct current (1 s every 30 s) for 15 min 30 min after electrical stimulation the animals were decapitated and brain was immediately removed on -20℃ ice plate and kept in -70℃ liquid nitrogen for determination of c fos mRNA expression in cerebral cortex, hypothalamus and hippocampus At the same time 4 ml of blood was collected from trunk for determination of ACTH and cortisol concentrations by immunoradiometric assay Results Plasma ACTH and cortisol levels and c fos mRNA expression in cerebral cortex, hypothalamus and hippocampus increased significantly in group S as compared with those in group C (P0 05).Conclusions The c fos gene is involved in molecular modulation of stress responses Propofol produces different effects on c fos gene expression in different brain regions
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Objective To investigate the effect of propofol on C-fos expression and glutamate concentration in rat cerebral cortex induced by ketamine. Methods Twenty-eight male Wistar rats weighing 260-280 g were randomly divided into four groups of seven animals: group 1 received normal saline intraperitoneally (ip) (group NS); group 2 received NS + ketamine 100mg?kg-1 ip (group K); group 3 received propofol 100 mg?kg-1 + ketamine 100mg?kg-1 ip (group PK); group 4 received diazepam 10mg?kg-1 + ketamine 100 mg?kg-1 ip (group DK). The interval between the two intraperitoneal injections was 5 min in each group. The animals were decapitated 30 min after ip injection. C-fos mRNA expression was detected by RT-PCR method and fos protein expression by immuno-histochemical technique. Another forty Wistar rats were randomly divided into 4 groups of 10 animals as was described above. Two hours after ip injection, five animals in each group were decapitated for microscopic examination and the other five animals for determination of water and glutamate content of cerebral cortex.Results C-fos mRNA expression increased at 30 min after intraperitoneal ketamine. Ketamine induced significant increase in Fos protein expression, and glutamate and water content in cerebral cortex 2 h after ip injection. Propofol and diazepam inhibited the increases induced by ketamine ( P
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Object To establish a tissue culture and rapid propagation system for the medicinal plant Iphigenia indica Kunth. Methods Clusters of seedlings and protocorms and induction were studied on MS media with different parts of the plant, such as corms, stems, leaves and root tips as explants. Results The proper media for protocorm inducing, propagation and rooting were found in this paper, rapid propagation system of I. indica was established and the plant could put in large scale production. Conclusion Sprout can be successfully induced from corm and stem on MS media. MS+6-BA 2 mg/L+NAA 0.5 mg/L can induce both sprout and protocorm, and fit for large scale production. 1/2 MS+IBA 0.5 mg/L+NAA 0.2 mg/L is good for rooting.
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Objective To detemine the effects of ketamine on c fos gene expression in the different brain regions of rats following stress responses Methods Twenty one male Wistar rats were randomly allocated to receiving intraperitoneally 2 ml normal saline (group C or group S,n=7) or ketamine 100mg?kg 1 (group K,n=7) respectively, 5 min later, the stress response was induced with electrical foot shock (2 0mA,1s of duration,once every 30s,over 15min)only in group S and K All rats were decapitated 30min after the stress to extract the total cellular RNA of cerebral cortex, hypothalamus and hippocampus RT PCR technique was applied to determining cDNA amplification products with ? actin mRNA being as an internal control Densities of DNA bands were quantified using image analysis system Results The c fos mRNA levels were markedly elevated in group S as compared with control levels (P0 05).Conclusions The c fos gene involves in molecular modulation of stress responses Ketamine produce different effects on the expression of c fos gene in the different brain regions